pBabepuroBAF180
(Plasmid #41078)

• Depositing Lab: Ramon Parsons
• Publication: Xia et al Cancer Res. 2008 Mar 15;68(6):1667-74.

Backbone

• Vector backbone: pBabe-puro
• Backbone manufacturer: Weinberg lab (Addgene Plasmid #1764)
• Backbone size w/o insert (bp): 5169
• Total vector size (bp): 10130
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: To avoid new spontaneous mutations, we try to harvest the bacteria for DNA in the late log or early saturation phase of growth (after having selected individual colonies).
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BAF180
• Alt name: PBRM1
• Alt name: PB1
• Species: H. sapiens (human)
• Insert Size (bp): 4961
• Mutation: 3 silent mutations: 3595 a to g; 4981 a to t; 5875 a to g
• GenBank ID: NM_018313.4
• Entrez Gene: PBRM1 (a.k.a. BAF180, PB1, RCC, SMARCH1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry