|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||41845||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6142
- Total vector size (bp) 6970
Vector typeYeast Expression
Growth in Bacteria
Growth Strain(s)XL1 Blue
Copy numberLow Copy
Insert Size (bp)765
/ Fusion Proteins
- HA (N terminal on backbone)
- c-myc (C terminal on backbone)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCT302 was a gift from Dane Wittrup (Addgene plasmid # 41845 ; http://n2t.net/addgene:41845 ; RRID:Addgene_41845)
For your References section:Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody. Midelfort KS, Hernandez HH, Lippow SM, Tidor B, Drennan CL, Wittrup KD. J Mol Biol. 2004 Oct 22;343(3):685-701. 10.1016/j.jmb.2004.08.019 PubMed 15465055