Purpose(Empty Backbone) expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||73447||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
- Promoter GAL1
/ Fusion Proteins
- AGA2 (N terminal on backbone)
- T7 peptide (N terminal on backbone)
- Xpress tag (N terminal on backbone)
- V5 tag (C terminal on backbone)
- 6xHis (C terminal on backbone)
Growth in Bacteria
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYD1 was a gift from Dane Wittrup (Addgene plasmid # 73447 ; http://n2t.net/addgene:73447 ; RRID:Addgene_73447)
For your References section:Isolation of anti-T cell receptor scFv mutants by yeast surface display. Kieke MC, Cho BK, Boder ET, Kranz DM, Wittrup KD. Protein Eng. 1997 Nov;10(11):1303-10. PubMed 9514119
Map uploaded by the depositor.