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pBbA5c-MevT(CO)-MBIS(CO, ispA)
(Plasmid #35151)


Item Catalog # Description Quantity Price (USD)
Plasmid 35151 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Species
    S. cerevisiae (budding yeast); E. Coli
  • Mutation
    R364S in Mevalonate Kinase
  • Promoter LacUV5,

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1, BglII (not destroyed)
  • 3′ cloning site BamH1, Xho1 (not destroyed)
  • 5′ sequencing primer gatcgtttaggcacccca
  • 3′ sequencing primer CGTTTTATTTGATGCCTGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the R364 in Mevalonate Kinase (MK) may be susceptible to mutations. The R364S mutation is not expected to impact overall function, however the exact effects are unknown.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbA5c-MevT(CO)-MBIS(CO, ispA) was a gift from Jay Keasling & Taek Soon Lee (Addgene plasmid # 35151 ; ; RRID:Addgene_35151)
  • For your References section:

    Identification and microbial production of a terpene-based advanced biofuel. Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, Lee TS. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. 10.1038/ncomms1494 PubMed 21952217