|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31230||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6247
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Insert Size (bp)1171
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe phage-UbC RIG vector for lentiviral expression was a gift from G. Mostoslavsky and G. Vainer (Harvard University). Mostoslavsky, G., Fabian, A.J., Rooney, S., Alt, F.W. & Mulligan, R.C. Complete correction of murine Artemis immunodeficiency by lentiviral vector-mediated gene transfer. Proc. Natl. Acad. Sci. USA 103, 16406–16411 (2006).
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
Additional reference: Lionnet T, Czaplinski K, Darzacq X, Shav-Tal Y, Wells AL, Chao JA, Park HY, de Turris V, Lopez-Jones M, Singer RH. A transgenic mouse for in vivo detection of endogenous labeled mRNA. Nat Methods. Feb;8(2):165-70 (2011)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:UbC NLS-HA-MCP-YFP was a gift from Robert Singer (Addgene plasmid # 31230 ; http://n2t.net/addgene:31230 ; RRID:Addgene_31230)
For your References section:In vivo imaging of labelled endogenous I. Grunwald D, Singer RH. Nature. 2010 Sep 30. 467(7315):604-7. 10.1038/nature09438 PubMed 20844488