327,147 results

1. pYLCRISPR/Cas9Pubi-B

   Plasmid

   #66188

   Purpose

   expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Plant

   Mutation

   plant-codon optimized with higher GC contents (62…

   Promoter

   maize ubiquitin promoter (Pubi)

   Available since

   Sept. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-SFPQ

   Plasmid

   #166960

   Purpose

   Expresses FLAG-tagged SFPQ protein in pcDNA3.1

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ (SFPQ Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-SFPQ

   Plasmid

   #166960

   Purpose

   Expresses FLAG-tagged SFPQ protein in pcDNA3.1

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ (SFPQ Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV5)

   Viral Prep

   #105530-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CMV

   Tags

   EGFP

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pET-dCas9-VP64-6xHis

   Plasmid

   #62935

   Purpose

   Expression of dCas9-VP64-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   dCas9-VP64
   

   Use

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A and H840A amino acid changes render Cas9 nuc…

   Promoter

   T7

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-dCas9-VP64-6xHis

   Plasmid

   #62935

   Purpose

   Expression of dCas9-VP64-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   dCas9-VP64
   

   Use

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A and H840A amino acid changes render Cas9 nuc…

   Promoter

   T7

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-GFP-Gal8

   Plasmid

   #127191

   Purpose

   Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

   Depositor

   Jordan Green

   Article

   Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

   Insert

   GFP-Gal8 (LGALS8 Human)
   

   Use

   Tags

   Gal8 is fused to the c-terminus of GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-GFP-Gal8

   Plasmid

   #127191

   Purpose

   Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

   Depositor

   Jordan Green

   Article

   Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

   Insert

   GFP-Gal8 (LGALS8 Human)
   

   Use

   Tags

   Gal8 is fused to the c-terminus of GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. B95(DE3) ΔA ΔfabR ΔserB

   Bacterial Strain

   #197655

   Purpose

   This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

   Depositor

   Richard Cooley, Ryan Mehl

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Bacterial Resistance

   None

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

10. pLX-TRE-dCas9-KRAB-MeCP2-BSD

    Plasmid

    #140690

    Purpose

    Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    Cas9m4-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX-TRE-dCas9-KRAB-MeCP2-BSD

    Plasmid

    #140690

    Purpose

    Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    Cas9m4-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-Con/Foff 2.0-GCaMP6M (AAV8)

    Viral Prep

    #137120-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-GCaMP6M (#137120). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-GCaMP6M plasmid DNA. EF1a-driven expression of GCaMP6m in the presence of Cre recombinase (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Tags

    None

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

13. pLenti-mCherry-Mango II x 24

    Plasmid

    #127587

    Purpose

    Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

    Depositor

    David Rueda

    Article

    Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

    Insert

    mCherry-Mango II x24
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE Tet ON

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-mCherry-Mango II x 24

    Plasmid

    #127587

    Purpose

    Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

    Depositor

    David Rueda

    Article

    Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

    Insert

    mCherry-Mango II x24
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE Tet ON

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lamp1-mScarlet-I

    Plasmid

    #98827

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Lamp1
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lamp1-mScarlet-I

    Plasmid

    #98827

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Lamp1
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Raf-1 GST RBD 1-149

    Plasmid

    #13338

    Depositor

    Channing Der

    Article

    Brtva et al J Biol Chem. 1995 Apr 28. 270(17):9809-1

    Insert

    Raf-1 (RAF1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. Raf-1 GST RBD 1-149

    Plasmid

    #13338

    Depositor

    Channing Der

    Article

    Brtva et al J Biol Chem. 1995 Apr 28. 270(17):9809-1

    Insert

    Raf-1 (RAF1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LRRK2-WT

    Plasmid

    #17609

    Depositor

    Ted Dawson

    Article

    West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LRRK2-WT

    Plasmid

    #17609

    Depositor

    Ted Dawson

    Article

    West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge