167,825 results

1. pUCmini-iCAP-AAV.MaCPNS1

   Plasmid

   #185136

   Purpose

   non-standard AAV2 rep-AAV-MaCPNS1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

   Insert

   Synthetic construct isolate AAV-MaCPNS1 VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTETRIS-cargo

   Plasmid

   #126033

   Purpose

   Luciferase reporter construct for the study of cis-repressive activity in long noncoding RNAs. Also confers puromycin resistance.

   Depositor

   Mauro Calabrese

   Article

   Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

   Type

   Empty backbone

   Use

   Luciferase; Piggybac

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-CAG-jGCaMP8f-WPRE (AAV1)

   Viral Prep

   #179254-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from AAV-CAG-jGCaMP8f-WPRE (#179254). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8f-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   CAG

   Available Since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

4. pT077

   Plasmid

   #137879

   Purpose

   Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. hiActTS-GR

   Plasmid

   #79775

   Purpose

   high FRET control for ActTS-GR

   Depositor

   Tetsuya Kitaguchi

   Article

   Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

   Insert

   hiActTS-GR
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. OTUB1

   Plasmid

   #25149

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   otub1.040.271 (OTUB1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   amino acid residues 40-271

   Available Since

   Aug. 6, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

7. psPax2-D64V-NC-mNeon

   Plasmid

   #196509

   Purpose

   mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   mNeon
   

   Tags

   mNeon

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PKC epsilon WT

   Plasmid

   #21240

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC epsilon (Prkce Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

9. TFORF1331

   Plasmid

   #142785

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TFAP2B (TFAP2B Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. plc242-GFP-WDR24

    Plasmid

    #87050

    Purpose

    mammalian expression of WDR24

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. COC053

    Plasmid

    #209131

    Purpose

    Enzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.

    Depositor

    Christopher A. O'Callaghan

    Article

    O'callaghan et al Anal Biochem. 1999 Jan 1;266(1):9-15. do

    Insert

    Enzyme BirA (birA E. coli)
    

    Expression

    Bacterial

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. TP53-KO_gRNA_1

    Plasmid

    #195130

    Purpose

    TP53-targeting gRNA in the LRG2.1 backbone

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    TP53 KO gRNA 1 (TP53 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-SIRT2-Flag

    Plasmid

    #102624

    Purpose

    Expresses full length SIRT2 isoform 1 in mammalian cells

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    SIRT2 (SIRT2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry- SEC61B

    Plasmid

    #121160

    Purpose

    Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    SEC61B (SEC61B )
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJJS002

    Plasmid

    #188325

    Purpose

    PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::GFP::mIAA7
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AcpP pet29a C-terminal His Tag

    Plasmid

    #75016

    Purpose

    Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase

    Depositor

    Michael Burkart

    Article

    Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

    Insert

    AcpP (acpP E. coli)
    

    Tags

    His Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

17. p3HA-hMIB2

    Plasmid

    #33312

    Depositor

    Vanessa Redecke

    Article

    Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

    Insert

    MIB2 (MIB2 Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

18. Kras-Src FRET biosensor

    Plasmid

    #78302

    Purpose

    FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

    Insert

    Src biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCBL101

    Plasmid

    #199722

    Purpose

    A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene.

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    NPTII
    

    Expression

    Plant

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-Ef1a-hMGT-PGK-puro

    Plasmid

    #172394

    Purpose

    Lentiviral expression vector for human direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

    Insert

    Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
    

    Use

    Lentiviral

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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