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Cyclin D activates the Rb tumor suppressor by mono-phosphorylation.
Narasimha AM, Kaulich M, Shapiro GS, Choi YJ, Sicinski P, Dowdy SF
Elife. 2014 May 29:e02872. doi: 10.7554/eLife.02872.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
58905pCMV HA hRB-wtmammalian expression of wild type human Rb
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58906pCMV HA hRb delta CDKmammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed
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58907pCMV HA hRb delta CDK + S230mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restored
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58908pCMV HA hRB delta CDK + S249mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S249 restored
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58910pCMV HA hRB delta CDK + T252mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restored
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58911pCMV HA hRB delta CDK + T356mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T356 restored
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58912pCMV HA hRB delta CDK + T373mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored
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58913pCMV HA hRB delta CDK + S608mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restored
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58914pCMV HA hRB delta CDK + S612mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restored
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58915pCMV HA hRB delta CDK + S780mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S780 restored
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58916pCMV HA hRB delta CDK + S788mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S788 restored
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58918pCMV HA hRB delta CDK + S807mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S807 restored
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58919pCMV HA hRB delta CDK + S811mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S811 restored
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58920pCMV HA hRB delta CDK + T821mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T821 restored
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58921pCMV HA hRB delta CDK + T826mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T826 restored
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