ePop
(Plasmid #50953)

• Purpose: Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations
• Depositing Lab: Lingchong You
• Publication: Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi: 10.1371/journal.pone.0011909.

Backbone

• Vector backbone: pProTet.E
• Backbone manufacturer: Clontech
• Modifications to backbone: A 40bp oligo with NdeI and NheI sites was introduced into the AatII site of pProTet.E

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Please screen at least 5 small colonies by restriction digest immediately upon receipt. Plasmid is likely unstable.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: E lysis protein
• Species: phage φX174
• Insert Size (bp): 276

Gene/Insert 2

• Gene/Insert name: LuxI
• Species: V. fischeri
• Insert Size (bp): 582
• Mutation: mutated luxR
• GenBank ID: AY275714.1

Gene/Insert 3

• Gene/Insert name: LuxR
• Species: Aliivibrio fischeri
• Insert Size (bp): 753
• Mutation: Early stop codon introduced in mutated luxR results in loss of luxR functionality; the regulation of this gene is not required for oscillating behavior observed in the study
• GenBank ID:

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expression strain: MC4100