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pTD103luxI_sfGFP
(Plasmid #48885)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48885 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    LB should be supplemented with 0.2% glucose *
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LuxI, sfGFP, and LuxR
  • Promoter pLux
  • Tag / Fusion Protein
    • ssrA tag (AANDENYALAA) (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

* The presence of glucose will reduce the amount of expression from the Lux promoter driving GFP and other genes. Omitting the glucose could potentially make the cell overburdened.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTD103luxI_sfGFP was a gift from Jeff Hasty (Addgene plasmid # 48885 ; http://n2t.net/addgene:48885 ; RRID:Addgene_48885)
  • For your References section:

    A sensing array of radically coupled genetic 'biopixels'. Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722. 10.1038/nature10722 PubMed 22178928