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pYLL201
(Plasmid #53454)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53454 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 9045
  • Modifications to backbone
    DNA sequence deleted between Cla1 and Xba1 in Polylinker 1.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b)
  • Alt name
    HA-mTop2b
  • Alt name
    Top2b
  • Alt name
    topoisomerase (DNA) II beta
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4873
  • GenBank ID
    NM_009409
  • Entrez Gene
    Top2b (a.k.a. D230016L12Rik, Top-2)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Cla1 (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6 primer
  • 3′ sequencing primer T7 primer
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The full length mTop2b cDNA was amplified by RT-PCR using the total RNA isolated from the whole brain of E16.5 mouse embryo. The 5' forward primer contains the Cla1 restriction site and the HA tag, and the 3' reverse primer contains the Xba1 restriction site.

Addgene QC sequencing found an A253V mutation, it should not effect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYLL201 was a gift from Leroy Liu & Lisa Lyu (Addgene plasmid # 53454 ; http://n2t.net/addgene:53454 ; RRID:Addgene_53454)
  • For your References section:

    Activation of a novel ubiquitin-independent proteasome pathway when RNA polymerase II encounters a protein roadblock. Ban Y, Ho CW, Lin RK, Lyu YL, Liu LF. Mol Cell Biol. 2013 Oct;33(20):4008-16. doi: 10.1128/MCB.00403-13. Epub 2013 Aug 12. 10.1128/MCB.00403-13 PubMed 23938298