pEGFP-mNFM
(Plasmid #32909)

• Purpose: Mammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of mouse neurofilament protein M
• Depositing Lab: Anthony Brown
• Depositing Organization: Ohio State University
  Located in United States of America
• Publication: Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):299-309.
• Citations: 3 Articles

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4041
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP-mouse NFM fusion
• Alt name: EGFP-mNFM
• Alt name: neurofilament protein M
• Species: M. musculus (mouse)
• Insert Size (bp): 3339
• GenBank ID:
• Entrez Gene: Nefm (a.k.a. NF-M, NF160, NF165, Nef3, Nfm)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: multiple primers
• 3′ sequencing primer: multiple primers

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid first described in Yan et al. (2007) "The Polypeptide Composition of Moving and Stationary Neurofilaments in Cultured Sympathetic Neurons" Cell Motility and the Cytoskeleton 64:299–309.

Mouse NFM cDNA sequence deposited in Genbank (Accession number DQ201636).

DNA (Catalog # 32909-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 32909-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.