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(Plasmid #32907)


Item Catalog # Description Quantity Price (USD)
Plasmid 32907 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4134
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    EGFP-rat NFM fusion
  • Alt name
  • Alt name
    neurofilament protein M
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • Entrez Gene
    Nefm (a.k.a. Nef3, Nfm)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ApaI (not destroyed)
  • 3′ cloning site ApaI (destroyed during cloning)
  • 5′ sequencing primer multiple primers
  • 3′ sequencing primer multiple primers
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Rat NFM cDNA originally cloned by Ron Liem, Department of Pathology, Columbia University College of Physicians and Surgeons, New York
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid first described in Wang et al. (2000) "Rapid movement of axonal neurofilaments interrupted by prolonged pauses" Nature Cell Biology 2:137-141.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-rNFM was a gift from Anthony Brown (Addgene plasmid # 32907 ; ; RRID:Addgene_32907)
  • For your References section:

    Rapid movement of axonal neurofilaments interrupted by prolonged pauses. Wang L, Ho CL, Sun D, Liem RK, Brown A. Nat Cell Biol. 2000 Mar;2(3):137-41. 10.1038/35004008 PubMed 10707083