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Stoichiometry and assembly of mTOR complexes revealed by single-molecule pulldown.
Jain A, Arauz E, Aggarwal V, Ikon N, Chen J, Ha T
Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17833-8. doi: 10.1073/pnas.1419425111. Epub 2014 Dec 1.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
73384peYFP-C1-mTORExpresses YFP tagged mTOR
73385pRK5-HA-YFP-raptorExpresses YFP and HA tagged Human raptor
73386pRK5-HA-mCherry-raptorExpresses raptor tagged with mCherry and HA
73387pRK5-HA-YFP-rictorExpresses HA and YFP tagged Rictor
73388pCDNA-mSin1(1.1)-HAExpresses mSin-HA
73389pRK5-HA-YFP-PRAS40Expresses HA and YFP tagged PRAS40
73390pRK5-HA-YFP-DEPTORExpresses HA and YFP tagged DEPTOR

Antibodies from Article