GFP-AHPH-DM
(Plasmid #71368)

• Purpose: Location sensor for RhoA-GTP
• Depositing Lab: Alpha Yap
• Publication: Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93. doi: 10.1038/ncb3239. Epub 2015 Sep 14.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: C terminus of Anillin
• Species: H. sapiens (human)
• Insert Size (bp): 1450
• Mutation: A740D and E758K
• GenBank ID: 54443
• Entrez Gene: ANLN (a.k.a. FSGS8, Scraps, scra)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Michael Glotzer, University of Chicago
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Adapted AHPH domain derived from C-terminus of Anillin is used as a sensor for active RhoA (RhoA-GTP). GFP-AHPH-DM, which harbors two mutations (A740D and E758K) in its RBD domain, is used as a negative control with GFP-AHPH-WT.