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pEGFP-RhoA Biosensor
(Plasmid #68026)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 68026 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6140
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Anillin (AHD+PH)
  • Alt name
    RhoA Biosensor
  • Alt name
    AHPH
  • Alt name
    AHDPH
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1428
  • Entrez Gene
    ANLN (a.k.a. FSGS8, Scraps, scra)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer CATGGACGAGCTGTACAAG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-RhoA Biosensor was a gift from Michael Glotzer (Addgene plasmid # 68026 ; http://n2t.net/addgene:68026 ; RRID:Addgene_68026)
  • For your References section:

    Anillin is a scaffold protein that links RhoA, actin, and myosin during cytokinesis. Piekny AJ, Glotzer M. Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2007 Dec 27. 10.1016/j.cub.2007.11.068 PubMed 18158243