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Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells.
Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel H, Radbruch A, Krause E, Baumgrass R
J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
74046pMSCV-FLAG-hIKAROS-IRES-GFPretroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1
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74047pMSCV-FLAG-hHelios-IRES-GFPretroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1
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74049pMIG-hNFATc1/bCretroviral expression plasmid for human NFATc1/bC
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74050pMIG-hNFATc2retroviral expression plasmid for human NFATc2/C
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74051pMIG-hH4-AVITEVretroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage site
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74052pMSCV-AVITEV-GFPretroviral expression plasmid for GFP with N-terminal BirA-biotinylation signal and TEV celavage site
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74053pMIG-hNFATc2/C(RIT)-FLAG-AVITEVretroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site
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74054pMIG-hNFATc2/C(1-686)AVITEVretroviral expression plasmid for human NFATc2/C (without C-terminal region) with C-terminal BirA-biotinylation signal and TEV celavage site
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74055pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEVretroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site
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74056pMSCV-BirA-P2A-mCherryretroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion protein
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74057pMIG-AVITEV- hNFATc1/aA retroviral expression plasmid for human NFATc1/aA with N-terminal BirA-biotinylation signal and TEV celavage site
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74058pMIG-hNFATc2/C - AVITEVretroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage site
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74059pMIG-hNFATc1/bC - AVITEVretroviral expression plasmid for human NFATc1/bC with C-terminal BirA-biotinylation signal and TEV celavage site
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75232NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)CRISPR/Cas9 plasmid against human NFATc2
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75233NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)CRISPR/Cas9 plasmid against human NFATc2
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75234NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)
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75235NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)
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75236NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)CRISPR/Cas9 plasmid against human NFATc1
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75237NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)CRISPR/Cas9 plasmid against human NFATc1
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75238NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)
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75239NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)
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75240Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)CRISPR/Cas9 plasmid against human Ikaros
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75241Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)CRISPR/Cas9 plasmid against human Ikaros
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75242Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)
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75243Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)CRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)
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75244Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)CRISPR/Cas9 plasmid against human Helios
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75246Helios-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)CRISPR/Cas9 NICKASE plasmid against human Helios (1/2)
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75247Helios-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)CRISPR/Cas9 NICKASE plasmid against human Helios (2/2)
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75248pQCXIX_sh_hBcl10_#1retroviral shRNA vector against human BCL10, expresses GFP for transduction control
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75252pQCXIX_sh_HOIP/RNF31_#6retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control
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75253pQCXIX_sh_hBcl10_#16retroviral shRNA vector against human BCL10, expresses GFP for transduction control
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75254pQCXIX_sh_hBcl10_#2retroviral shRNA vector against human BCL10, expresses GFP for transduction control
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75255pQCXIX_sh_hMIB2_#9retroviral shRNA vector against human MIB2, expresses GFP for transduction control
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75256pMSCV40-EF1a-hBCL10-SOretroviral expression vector for expression of StrepOne-Tagged human BCL10
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75257pMSCV40-EF1a-hBCL10(1-114)-SOretroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-116)
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75258pMSCV40-EF1a-hBCL10(1-185)-SOretroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-185)
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