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(Plasmid #74057)


Item Catalog # Description Quantity Price (USD)
Plasmid 74057 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 7200
  • Total vector size (bp) 9320
  • Modifications to backbone
    added SV40-ORI
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
    human NFATc1, isoform alpha-A
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    mutation G1157A [R235Q] and the silent mutation C1980T, as already described in the original publication Northrop, J. P. et al. Nature 369, 497–502 (1994).
  • GenBank ID
  • Entrez Gene
    NFATC1 (a.k.a. NF-ATC, NF-ATc1.2, NFAT2, NFATc)
  • Promoter pMSCV-LTRs
  • Tag / Fusion Protein
    • AVI-TEV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SwaI, SnaB1 (not destroyed)
  • 3′ cloning site Sgf1 (not destroyed)
  • 5′ sequencing primer CGT TCG ACC CCG CCT CGA TCC
  • 3′ sequencing primer AGCTTGATATCGAATTCCG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The Avi-Tev-Tag is biotinylated by BirA E.coli biotin ligase when coexpressed, and can be cleaved by TEV protease.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIG-AVITEV- hNFATc1/aA was a gift from Ria Baumgrass (Addgene plasmid # 74057 ; ; RRID:Addgene_74057)
  • For your References section:

    Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel H, Radbruch A, Krause E, Baumgrass R. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326. 10.1074/jbc.M116.739326 PubMed 27637333