ATG-2460
(Plasmid #108713)

• Purpose: EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)
• Depositing Lab: Keith Wood
• Publication: Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10.1038/s41467-017-02542-9.

Backbone

• Vector backbone: pCDH-EF1-T2A-copGFP
• Backbone manufacturer: Systembio
• Backbone size w/o insert (bp): 6460
• Total vector size (bp): 8917
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CBR2opt-copGFP fusion
• Species: Pyrophorus plagiophthalamus
• Insert Size (bp): 2456
• Mutation: Promega used directed evolution to create CBR (Click Beetle Red). CBR2 contains 2 additional mutations (R344S+G351R) This gene is also codon optimized for mouse expression
• Promoter: elongation factor-1
• Tag / Fusion Protein:
  • CBR2opt fused to copGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: Not1 (unknown if destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Promega
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • CBR2 Limited Use Label License
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry