ATG-1929
(Plasmid
#108712)
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PurposeCBR2opt in pF4Ag expression vector with T7 and CMV promoter
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Depositing Lab
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Depositing OrganizationPromega Corporation
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108712 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 108712-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 108712-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
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Backbone
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Vector backbonepF4Ag
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3820
- Total vector size (bp) 5449
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCBR2opt
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SpeciesPyrophorus plagiophthalamus
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Insert Size (bp)1629
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MutationPromega used directed evolution to create CBR (Click Beetle Red). CBR2 contains 2 additional mutations (R344S+G351R) This gene is also codon optimized for mouse expression
- Promoter T7, CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sgf1 (not destroyed)
- 3′ cloning site Pme1 (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPromega
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 108712-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 108712-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ATG-1929 was a gift from Keith Wood (Addgene plasmid # 108712 ; http://n2t.net/addgene:108712 ; RRID:Addgene_108712) -
For your References section:
Click beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging. Hall MP, Woodroofe CC, Wood MG, Que I, Van't Root M, Ridwan Y, Shi C, Kirkland TA, Encell LP, Wood KV, Lowik C, Mezzanotte L. Nat Commun. 2018 Jan 9;9(1):132. doi: 10.1038/s41467-017-02542-9. 10.1038/s41467-017-02542-9 PubMed 29317625
Map uploaded by the depositor.
