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Complex transcriptional modulation with orthogonal and inducible dCas9 regulators.
Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. PubMed Article

Plasmids from Article

ID Plasmid Purpose
84239pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9Expresses ABA-inducible VPR-Sp dCas9
84240pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9Expresses GA-inducible VPR-Sp dCas9
84241pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9Expresses ABA-inducible KRAB-Sp dCas9
84242pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9Expresses GA-inducible KRAB-Sp dCas9
84243pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9Expresses ABA-inducible VPR-Sa dCas9
84244pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9Expresses GA-inducible VPR-Sa dCas9
84245pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9Expresses direct fusion KRAB-Sp dCas9
84246pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9Expresses direct fusion VPR-Sa dCas9
84247pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9Expresses direct fusion VPR-Sp dCas9
84248pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherryExpresses Sp sgTRE3G gRNA with a mCherry fluorescent marker
84249pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherryExpresses Sp sgSV40 gRNA with a mCherry fluorescent marker
84250pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherryExpresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker
84251pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFPExpresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker
84252pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFPExpresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker
84253pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFPExpresses Sp sgCD95-3 gRNA with an EGFP fluorescent marker
84254pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFPExpresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker
84255pSLQ2853-4 pHR: U6-Sasgv2CXCR4-2 CMV-EGFPExpresses optimized Sa sgCXCR4-2 gRNA with an EGFP fluorescent marker
84256pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFPExpresses optimized Sa sgCXCR4-3 gRNA with an EGFP fluorescent marker
84257pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABIExpresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation
84258pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherryExpresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate
84260pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherryExpresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation
84261pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9Expresses AND gate ABA/GA-inducible VPR-Sp dCas9
106895pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABIExpresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation

Antibodies from Article