LuTHy: a double‐readout bioluminescence‐based two‐hybrid technology for quantitative mapping of protein–protein interactions in mammalian cells
Trepte P, Kruse S, Kostova S, Hoffmann S, Buntru A, Tempelmeier A, Secker C, Diez L, Schulz A, Klockmeier K, Zenkner M, Golusik S, Rau K, Schnoegl S, Garner CC, Wanker EE.
Molecular Systems Biology (2018) 14, e8071 Article
Trepte P, Kruse S, Kostova S, Hoffmann S, Buntru A, Tempelmeier A, Secker C, Diez L, Schulz A, Klockmeier K, Zenkner M, Golusik S, Rau K, Schnoegl S, Garner CC, Wanker EE.
Molecular Systems Biology (2018) 14, e8071 Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 113442 | pcDNA3.1 NL | NanoLuc control expression vector |
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| 113443 | pcDNA3.1 PA-mCit | ProteinA-mCitrine control expression vector |
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| 113444 | pcDNA3.1 PA-mCit-NL | ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control) |
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| 113445 | pcDNA3.1 PA-NL | ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization) |
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| 113446 | pcDNA3.1 myc-NL-GW | NanoLuc Gateway shuttle vector for N-terminal fusions |
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| 113447 | pcDNA3.1 GW-NL-myc | NanoLuc Gateway shuttle vector for C-terminal fusions |
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| 113448 | pcDNA3.1 PA-mCit-GW | ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions |
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| 113449 | pcDNA3.1 GW-mCit-PA | ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions |
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| 113450 | pLenti NL | Lentiviral NanoLuc control expression vector |
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| 113451 | pLenti PA-mCit | Lentiviral ProteinA-mCitrine expression vector |
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| 113452 | pLenti PA-mCit-NL | Lentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control) |
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| 113453 | pLenti PA-NL | Lentiviral ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization) |
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| 113454 | pLenti myc-NL-GW | Lentiviral NanoLuc Gateway shuttle vector for N-terminal fusions |
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| 113455 | pLenti GW-NL-myc | Lentiviral NanoLuc Gateway shuttle vector for C-terminal fusions |
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| 113456 | pLenti PA-mCit-GW | Lentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions |
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| 113457 | pLenti GW-mCit-PA | Lentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions |
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| 113458 | pcDNA3.1 NL-BCL2L1 | NanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD) |
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| 113459 | pcDNA3.1 PA-mCit-BAD | ProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1) |
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