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Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci.
Sabri S, Steen JA, Bongers M, Nielsen LK, Vickers CE
Microb Cell Fact. 2013 Jun 24;12(1):60.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
46763pKIKOarsBCmE.coli genomic integration at arsB
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46764pKIKOlacZCmE.coli genomic integration at lacZ
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46765pKIKOrbsARCmE.coli genomic integration rbsAR locus
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46766pKIKOarsBKmE.coli genomic integration at arsB
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46767pKIKOlacZKmE.coli genomic integration at lacZ
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46768pKIKOrbsARKmE.coli genomic integration rbsAR locus
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Antibodies from Article