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Addgene

Csy4H29AS50C
(Plasmid #45029)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45029 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pHM-GWA
  • Backbone size w/o insert (bp) 8236
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Csy4H29AS50C
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
    564
  • Mutation
    H29A mutation to abolish catalytic activity and Ser50Cys mutation to enable coupling to biotin
  • GenBank ID
    115585590 ABJ11605
  • Promoter T7
  • Tag / Fusion Protein
    • His6-MBP (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Csy4H29AS50C was a gift from Jennifer Doudna (Addgene plasmid # 45029 ; http://n2t.net/addgene:45029 ; RRID:Addgene_45029)
  • For your References section:

    RNA-protein analysis using a conditional CRISPR nuclease. Lee HY, Haurwitz RE, Apffel A, Zhou K, Smart B, Wenger CD, Laderman S, Bruhn L, Doudna JA. Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):5416-21. doi: 10.1073/pnas.1302807110. Epub 2013 Mar 14. 10.1073/pnas.1302807110 PubMed 23493562