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pHMGWA-Pa14Csy4
(Plasmid #41091)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41091 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHMGWA
  • Backbone size w/o insert (bp) 8236
  • Total vector size (bp) 7344
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Csy4
  • Alt name
    Pa14_33300
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
    564
  • Mutation
    Wild type
  • GenBank ID
    115585590 ABJ11605
  • Promoter T7
  • Tag / Fusion Protein
    • His6-MBP (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

For more information on Doudna Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/doudna/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHMGWA-Pa14Csy4 was a gift from Jennifer Doudna (Addgene plasmid # 41091 ; http://n2t.net/addgene:41091 ; RRID:Addgene_41091)
  • For your References section:

    Sequence- and structure-specific RNA processing by a CRISPR endonuclease. Haurwitz RE, Jinek M, Wiedenheft B, Zhou K, Doudna JA. Science. 2010 Sep 10;329(5997):1355-8. 10.1126/science.1192272 PubMed 20829488