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pCAG-dCas9-5xPlat2AflD

Plasmid

#82560

Purpose

Expression vector for Cas9 peptide array (linker length: 22aa).

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

dCas9-5xPlat2AflD

Expression

Mammalian

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-dCas9-5xPlat2AflD

Plasmid

#82560

Purpose

Expression vector for Cas9 peptide array (linker length: 22aa).

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

dCas9-5xPlat2AflD

Expression

Mammalian

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR/D_creERt2

Plasmid

#27321

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

creERt2

Use

Multisite gateway middle entry vector

Available Since

Jan. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR/D_creERt2

Plasmid

#27321

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

creERt2

Use

Multisite gateway middle entry vector

Available Since

Jan. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pSNAPf-Cox8A Control Plasmid

Plasmid

#101129

Purpose

Expression of SNAP-Tagged Cox8A in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Cox8A (COX8A Human)

Tags

SNAP-tag (SNAPf)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pKB01_mCherry

Plasmid

#69104

Purpose

Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pKB01_mCherry

Plasmid

#69104

Purpose

Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-ClpX

Plasmid

#234733

Purpose

Human-codon optimized E. coli ClpX in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

ClpX

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pBB540

Plasmid

#27393

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

grpE, clpB

Expression

Bacterial

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only

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pBB540

Plasmid

#27393

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

grpE, clpB

Expression

Bacterial

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only

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gRNA_Cloning Vector

Plasmid

#41824

Purpose

An empty gRNA expression vector, used to create a gRNA to a specific sequence.

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

AflII site for Gibson Assembly

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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gRNA_Cloning Vector

Plasmid

#41824

Purpose

An empty gRNA expression vector, used to create a gRNA to a specific sequence.

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

AflII site for Gibson Assembly

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-GFP11-Clathrin light chain

Plasmid

#70217

Purpose

Expresses GFP11-clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11-Clathrin light chain (CLTA Human)

Tags

GFP11

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-GFP11-Clathrin light chain

Plasmid

#70217

Purpose

Expresses GFP11-clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11-Clathrin light chain (CLTA Human)

Tags

GFP11

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBPnlsLexA::p65Uw

Plasmid

#26230

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

nlsLexA::p65 (RELA Human)

Expression

Insect

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pBPnlsLexA::p65Uw

Plasmid

#26230

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

nlsLexA::p65 (RELA Human)

Expression

Insect

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pmEmerald-ERGIC-53

Plasmid

#170717

Purpose

Expresses mEmerald-tagged human ERGIC-53 at high level in mammalian cells.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Insert

ERGIC-53 (LMAN1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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p11-LacY-wtx1

Plasmid

#69056

Purpose

The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

Depositor

Huimin Zhao

Article

Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

Inserts

ccdB (ccdB )
lacY (lacY )

Tags

A177C

Promoter

pBAD and pLac

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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p11-LacY-wtx1

Plasmid

#69056

Purpose

The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

Depositor

Huimin Zhao

Article

Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

Inserts

ccdB (ccdB )
lacY (lacY )

Tags

A177C

Promoter

pBAD and pLac

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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tetO.Nfia.Blast

Plasmid

#117270

Purpose

Expresses Nfia and blasticidin resistance gene under control of tetO sequence.

Depositor

Henrik Ahlenius

Article

Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

Insert

Nfia (Nfia Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tet-on

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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