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Addgene

pLUT7 HA-GLI1
(Plasmid #62970)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62970 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLUT7
  • Backbone size w/o insert (bp) 6517
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GLI1
  • Alt name
    GLI family zinc finger 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    GLI1 (a.k.a. GLI, PAPA8, PPD1)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (Blunt) (unknown if destroyed)
  • 3′ cloning site EcoRI (Blunt) (unknown if destroyed)
  • 5′ sequencing primer CMV E1
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLUT7 HA-GLI1 was a gift from Michael Ruppert (Addgene plasmid # 62970 ; http://n2t.net/addgene:62970 ; RRID:Addgene_62970)

  • For your References section:

    SOX9 inhibits beta-TrCP-mediated protein degradation to promote nuclear GLI1 expression and cancer stem cell properties. Deng W, Vanderbilt DB, Lin CC, Martin KH, Brundage KM, Ruppert JM. J Cell Sci. 2015 Jan 27. pii: jcs.162164. 10.1242/jcs.162164 PubMed 25632159
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