PurposeExpresses HA-tagged Gli1
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62967||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5169
Growth in Bacteria
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
Alt nameGLI family zinc finger 1
SpeciesH. sapiens (human)
Entrez GeneGLI1 (a.k.a. GLI, PAPA8, PPD1)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site SnaB I (blunt) (unknown if destroyed)
- 3′ cloning site SnaB I (blunt) (unknown if destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBABEPuro-HA-GLI1 was a gift from Michael Ruppert (Addgene plasmid # 62967 ; http://n2t.net/addgene:62967 ; RRID:Addgene_62967)
For your References section:SOX9 inhibits beta-TrCP-mediated protein degradation to promote nuclear GLI1 expression and cancer stem cell properties. Deng W, Vanderbilt DB, Lin CC, Martin KH, Brundage KM, Ruppert JM. J Cell Sci. 2015 Jan 27. pii: jcs.162164. 10.1242/jcs.162164 PubMed 25632159