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Addgene

paavCAG-JxON-pre-mGRASP-mCerulean
(Plasmid #51903)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51903 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    paavCAG-Jx
  • Backbone size w/o insert (bp) 5000
  • Vector type
    AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    presynaptic mGRASP
  • Alt name
    pre-mGRASP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1722
  • GenBank ID
    JN898961
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer gcggctctagagcctctgcta
  • 3′ sequencing primer ttaaagcagcgtatccacat
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that there may be a few sequence discrepancies between Addgene's NGS results for this plasmid and the reference map provided by the depositing lab. These changes are in the backbone region outside of the viral cassette and should not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    paavCAG-JxON-pre-mGRASP-mCerulean was a gift from Jinhyun Kim (Addgene plasmid # 51903 ; http://n2t.net/addgene:51903 ; RRID:Addgene_51903)
  • For your References section:

    Structured Synaptic Connectivity between Hippocampal Regions. Druckmann S, Feng L, Lee B, Yook C, Zhao T, Magee JC, Kim J. Neuron. 2014 Feb 5;81(3):629-40. doi: 10.1016/j.neuron.2013.11.026. Epub 2014 Jan 9. 10.1016/j.neuron.2013.11.026 PubMed 24412418