|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40248||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 7900
Vector typeCyanobacteria cloning vector
Growth in Bacteria
Bacterial Resistance(s)Spectinomycin & Streptomycin
Gene/Insert nameOne Step Cloneing Vector for Overexpression
SpeciesSynechococcus elongatus PCC 7942
- Promoter n/a
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
pAM2990 was partially digested with SacI and re-ligatedto remove the antihel fragment. The net result is the insertion of the BglII fragment from pAM2255 into BamHI digested pAM1303 in the orientation that Ptrc goes away from the SacI site in pAM1303 (SacI digestion gives a 1.7 KB band). This plasmid can be used as a one-step cloning vector for overexpression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAM2991 was a gift from Susan Golden (Addgene plasmid # 40248 ; http://n2t.net/addgene:40248 ; RRID:Addgene_40248)
For your References section:LdpA: a component of the circadian clock senses redox state of the cell. Ivleva NB, Bramlett MR, Lindahl PA, Golden SS. EMBO J. 2005 Mar 23;24(6):1202-10. Epub 2005 Mar 10. 10.1038/sj.emboj.7600606 PubMed 15775978