|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40239||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Total vector size (bp) 8400
Vector typeCyanobacteria cloning vector
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
Gene/Insert nameCm Resistance, neutral site II integration platform for Synechococcus
SpeciesSynechococcus elongatus PCC 7942
Insert Size (bp)1600
- Promoter n/a
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
cloning sites: NheI, XhoI, SmaI, XbaI, StuI, SalI, and EcoRV.
Chloramphenicol cassette from pUC1819 (Cm resistant) as a BamHI fragment into BglII site of pAM1572 such that it reads away from the MCS and a ScaI digest gives a 3.9kb band. NSII vector; unique cloning sites NheI, XhoI, SmaI, XbaI, StuI, SalI, EcoRV.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAM1573 was a gift from Susan Golden (Addgene plasmid # 40239 ; http://n2t.net/addgene:40239 ; RRID:Addgene_40239)
For your References section:Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Andersson CR, Tsinoremas NF, Shelton J, Lebedeva NV, Yarrow J, Min H, Golden SS. Methods Enzymol. 2000;305:527-42. PubMed 10812624