|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40237||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 8100
Vector typeCyanobacteria cloning vector
Growth in Bacteria
Gene/Insert namepromoterless luxAB reporter
SpeciesSynechococcus elongatus PCC 7942
Insert Size (bp)2400
- Promoter n/a
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Lux Genes: luxAB
cloning sites NotI and BamHI
Blunted (by Klenow) SalI-PvuII luxAB fragment (from pLAV1) was cloned in the SmaI site of pAM1303. SalI site is approximately 135bp upstream of luxA ATG within the luxD reading frame. You can clone promoters upstream of luxAB in NotI or BamHI sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAM1414 was a gift from Susan Golden (Addgene plasmid # 40237 ; http://n2t.net/addgene:40237 ; RRID:Addgene_40237)
For your References section:Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Andersson CR, Tsinoremas NF, Shelton J, Lebedeva NV, Yarrow J, Min H, Golden SS. Methods Enzymol. 2000;305:527-42. PubMed 10812624