pAM1954
(Plasmid #40250)

• Depositing Lab: Susan Golden
• Publication: Yoon et al Science. 1998 Oct 30;282(5390):935-8.

Backbone

• Vector backbone: pAM542
• Total vector size (bp): 11000
• Vector type: Cyanobacteria cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GFPmut2
• Species: Anabaena sp. Strain PCC 7120
• Insert Size (bp): 800
• Promoter: n/a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • GenBank File
  • Strain Database Information

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

gfp from pKEN2-GFPmut2 inserted into pAM542, which contains PrbcL on a shuttle vector.

HindIII-Klenow filled-BamHI fragment containing GFPmut2 was inserted into SacI-T4 blunted-BamHI digested pAM542. PrbcL promoter fragment drives GFPmut2.