pAM2314
(Plasmid #40246)

• Depositing Lab: Susan Golden
• Publication: Ditty et al Microbiology. 2005 Aug;151(Pt 8):2605-13.

Backbone

• Vector backbone: pAM1303
• Total vector size (bp): 5829
• Vector type: Cyanobacteria cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Polylinker
• Species: Synechococcus elongatus PCC 7942
• Insert Size (bp): 48
• Promoter: n/a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • GenBank File
  • Strain Database Information

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NS1 vector

cloning sites: NotI, SacII, BglII, SnaBI, BsiWI, MluI, EcoRI, XhoI, StuI, SpeI, and BamHI.

additional unique sites inserted between the NotI and BamHI sites of the NSI vector pAM1303:
5’-NotI-SacII-BglII-SnaBI-BsiWI-MluI-EcoRI-XhoI-StuI-SpeI-BamHI-3’