|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40240||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 8082
Vector typeCyanobacteria cloning vector
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Gene/Insert nameKm Resistance, neutral site II integration platform for Synechococcus
SpeciesSynechococcus elongatus PCC 7942
Insert Size (bp)1500
- Promoter n/a
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
cloning sites: NheI, XbaI, StuI, SalI, and EcoRV.
Km cassette into Klenow blunted BglII site of pAM1572. XhoI digest gives 0.7kb band. NSII vector; unique cloning sites NheI, XbaI, StuI, SalI, EcoRV.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAM1579 was a gift from Susan Golden (Addgene plasmid # 40240 ; http://n2t.net/addgene:40240 ; RRID:Addgene_40240)
For your References section:Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Andersson CR, Tsinoremas NF, Shelton J, Lebedeva NV, Yarrow J, Min H, Golden SS. Methods Enzymol. 2000;305:527-42. PubMed 10812624