Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #40243)


Item Catalog # Description Quantity Price (USD)
Plasmid 40243 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 5700
  • Vector type
    Cyanobacteria cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Sp/Sm Resistance, neutral site I integration platform for Synechococcus
  • Species
    Synechococcus elongatus PCC 7942
  • Insert Size (bp)
  • Promoter n/a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NS1 vector

Cloning sites: NotI, BamHI and SmaI.

insert: GATCGGCGGCCGCG .........
...........CCGCCGGCGCCTAG NotI can be used as a primer site. Transforms and conjugates as well as pAM1175. Put in BamHI site of pAM1290 ; keeps a unique BamHI site on right.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM1303 was a gift from Susan Golden (Addgene plasmid # 40243 ; ; RRID:Addgene_40243)
  • For your References section:

    mRNA stability is regulated by a coding-region element and the unique 5' untranslated leader sequences of the three Synechococcus psbA transcripts. Kulkarni RD, Golden SS. Mol Microbiol. 1997 Jun;24(6):1131-42. 10.1046/j.1365-2958.1997.4201768.x PubMed 9218763