|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40243||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Total vector size (bp) 5700
Vector typeCyanobacteria cloning vector
Growth in Bacteria
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
Gene/Insert nameSp/Sm Resistance, neutral site I integration platform for Synechococcus
SpeciesSynechococcus elongatus PCC 7942
Insert Size (bp)14
- Promoter n/a
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Cloning sites: NotI, BamHI and SmaI.
insert: GATCGGCGGCCGCG .........
...........CCGCCGGCGCCTAG NotI can be used as a primer site. Transforms and conjugates as well as pAM1175. Put in BamHI site of pAM1290 ; keeps a unique BamHI site on right.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAM1303 was a gift from Susan Golden (Addgene plasmid # 40243 ; http://n2t.net/addgene:40243 ; RRID:Addgene_40243)
For your References section:mRNA stability is regulated by a coding-region element and the unique 5' untranslated leader sequences of the three Synechococcus psbA transcripts. Kulkarni RD, Golden SS. Mol Microbiol. 1997 Jun;24(6):1131-42. 10.1046/j.1365-2958.1997.4201768.x PubMed 9218763