pAM2195
(Plasmid #40245)

• Depositing Lab: Susan Golden
• Publication: Mackey et al Methods Mol Biol. 2007;362:115-29.

Backbone

• Vector backbone: pAM1582
• Total vector size (bp): 16600
• Vector type: Cyanobacteria cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PpsbAI::luxCDE, PpsbAI::luxAB
• Species: Synechococcus elongatus PCC 7942
• Insert Size (bp): 5400
• Promoter: n/a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • Strain Database Information
  • GenBank File

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NSII.1 vector

Lux Genes: luxAB & luxCDE

synthesizes luciferase and substrate in S. elongatus

SmaI--PvuII fragment containing PpsbAI::luxCDE was cloned into SmaI site of pAM1582. Orientation of PpsbAI::luxCDE is same as that of PpsbAI::luxAB. NS2.1, PpsbAI::luxCDE, PpsbAI::luxAB plasmid.