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(Plasmid #40245)


Item Catalog # Description Quantity Price (USD)
Plasmid 40245 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    PpsbAI::luxCDE, PpsbAI::luxAB
  • Species
    Synechococcus elongatus PCC 7942
  • Insert Size (bp)
  • Promoter n/a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NSII.1 vector

Lux Genes: luxAB & luxCDE

synthesizes luciferase and substrate in S. elongatus

SmaI--PvuII fragment containing PpsbAI::luxCDE was cloned into SmaI site of pAM1582. Orientation of PpsbAI::luxCDE is same as that of PpsbAI::luxAB. NS2.1, PpsbAI::luxCDE, PpsbAI::luxAB plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM2195 was a gift from Susan Golden (Addgene plasmid # 40245 ; ; RRID:Addgene_40245)
  • For your References section:

    Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria. Mackey SR, Ditty JL, Clerico EM, Golden SS. Methods Mol Biol. 2007;362:115-29. 10.1007/978-1-59745-257-1_8 PubMed 17417005