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pAD48-daf-2 RNAi
(Plasmid #34834)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 34834 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2961
  • Modifications to backbone
    pBluescript was converted into pAD12, with the introduction of T7 promoters at both sides of MCS.
  • Vector type
    Bacterial Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
  • Entrez Gene
    daf-2 (a.k.a. CELE_Y55D5A.5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer M13F(-20)
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Andrew Dillin and Cynthia Kenyon, UCSF.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use HT115 bacteria for experiments--Grow O/N on LB supplemented with carbenicillin (100ug/ml) & tetracyclin (12.5ug/ml) and pick single colony for liquid culture.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAD48-daf-2 RNAi was a gift from Cynthia Kenyon (Addgene plasmid # 34834 ; ; RRID:Addgene_34834)
  • For your References section:

    Timing requirements for insulin/IGF-1 signaling in C. elegans. Dillin A, Crawford DK, Kenyon C. Science. 2002 Oct 25;298(5594):830-4. 10.1126/science.1074240 PubMed 12399591