Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34834||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2961
Modifications to backbonepBluescript was converted into pAD12, with the introduction of T7 promoters at both sides of MCS.
Vector typeBacterial Expression, RNAi
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
SpeciesC. elegans (nematode)
Insert Size (bp)2000
Entrez Genedaf-2 (a.k.a. CELE_Y55D5A.5)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer M13F(-20)
- 3′ sequencing primer M13R (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byAndrew Dillin and Cynthia Kenyon, UCSF.
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Use HT115 bacteria for experiments--Grow O/N on LB supplemented with carbenicillin (100ug/ml) & tetracyclin (12.5ug/ml) and pick single colony for liquid culture.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAD48-daf-2 RNAi was a gift from Cynthia Kenyon (Addgene plasmid # 34834 ; http://n2t.net/addgene:34834 ; RRID:Addgene_34834)
For your References section:Timing requirements for insulin/IGF-1 signaling in C. elegans. Dillin A, Crawford DK, Kenyon C. Science. 2002 Oct 25;298(5594):830-4. 10.1126/science.1074240 PubMed 12399591