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Purposekdrl promoter for Gateway cloning
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78687 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONRp4p1r
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2600
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namekdrl promoter
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Alt nameflk-1
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)6400
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Entrez Genekdrl (a.k.a. flk, flk-1, flk1, kdr, kdra, vegfr-2, vegfr2, vegfr2a, vegfr4, vegr2, wu:fk52c05)
- Promoter kdrl
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p5E-kdrl was a gift from Jau-Nian Chen & Sarah Childs (Addgene plasmid # 78687 ; http://n2t.net/addgene:78687 ; RRID:Addgene_78687) -
For your References section:
FoxH1 negatively modulates flk1 gene expression and vascular formation in zebrafish. Choi J, Dong L, Ahn J, Dao D, Hammerschmidt M, Chen JN. Dev Biol. 2007 Apr 15;304(2):735-44. Epub 2007 Jan 20. 10.1016/j.ydbio.2007.01.023 PubMed 17306248
