Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #70052)


Item Catalog # Description Quantity Price (USD)
Plasmid 70052 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 2765
  • Total vector size (bp) 4367
  • Vector type
    Gateway 5' Element

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    mfap4 promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13F (-21)
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Addgene's quality control sequencing has identified a number of single-nucleotide discrepancies, compared to the NCBI Genbank reference sequence for the Zebrafish mfap4 promoter. The depositing laboratory is aware of these differences, and states that they are polymorphisms. This plasmid accurately reflects the construct as it was used in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5E-mfap4 was a gift from David Tobin (Addgene plasmid # 70052 ; ; RRID:Addgene_70052)
  • For your References section:

    The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage Behavior during Mycobacterial Infection in Zebrafish. Walton EM, Cronan MR, Beerman RW, Tobin DM. PLoS One. 2015 Oct 7;10(10):e0138949. doi: 10.1371/journal.pone.0138949. eCollection 2015. PONE-D-15-31888 [pii] PubMed 26445458