|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26029||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2810
Vector typeEntry vector
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Gateway Cloning
- 5′ cloning site attL1 (not destroyed)
- 3′ cloning site attR2 (not destroyed)
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13rev (Common Sequencing Primers)
"p5E" refers to 5' entry clones flanked by L4-R1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:476 p5E-mcs was a gift from Nathan Lawson (Addgene plasmid # 26029 ; http://n2t.net/addgene:26029 ; RRID:Addgene_26029)
For your References section:Robust Identification of Developmentally Active Endothelial Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq. Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND. Cell Rep. 2017 Jul 18;20(3):709-720. doi: 10.1016/j.celrep.2017.06.070. 10.1016/j.celrep.2017.06.070 PubMed 28723572