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pDestTol2pA2-U6:gRNA
(Plasmid #63157)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63157 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDestTol2pA2 (Tol2Kit#394)
  • Backbone size (bp) 6354
  • Modifications to backbone
    Added a zebrafish U6 promoter driving a gRNA scaffold containing BseRI sites to clone the seed
  • Vector type
    CRISPR ; Tol2 destination vector for Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Cloning Information

Resource Information

Depositor Comments

Reference for the Tol2Kit:
Kwan, K.M., Fujimoto, E., Grabher, C., Mangum, B.D., Hardy, M.E., Campbell, D.S., Parant, J.M., Yost, H.J., Kanki, J.P., and Chien, C.B. (2007). The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Dev Dyn 236, 3088-3099.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDestTol2pA2-U6:gRNA was a gift from Leonard Zon (Addgene plasmid # 63157)
  • For your References section:

    A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish. Ablain J, Durand EM, Yang S, Zhou Y, Zon LI. Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 10.1016/j.devcel.2015.01.032. 10.1016/j.devcel.2015.01.032 PubMed 25752963