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A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish.
Ablain J, Durand EM, Yang S, Zhou Y, Zon LI
Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 10.1016/j.devcel.2015.01.032.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
63154pME-Cas9Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS
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63155pME-Cas9-T2A-GFPMiddle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.
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63156pDestTol2CG2-U6:gRNADestination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.
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63157pDestTol2pA2-U6:gRNADestination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.
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