Purpose(Empty Backbone) Multiple cloning site in 3' gateway entry vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49004||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2639
Vector typegateway 3' Entry
Growth in Bacteria
Gene/Insert nameMultiple Cloning Site
Insert Size (bp)26
- Cloning method Gateway Cloning
- 5′ sequencing primer m13F
- 3′ sequencing primer m13R (Common Sequencing Primers)
There is a single base mismatch between Addgene's quality control sequence and the full plasmid reference sequence. The change is in backbone region and should not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:599 p3E-mcs1 was a gift from Nathan Lawson (Addgene plasmid # 49004 ; http://n2t.net/addgene:49004 ; RRID:Addgene_49004)
For your References section:Post-transcriptional mechanisms contribute to Etv2 repression during vascular development. Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N. Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 10.1016/j.ydbio.2013.08.028. 10.1016/j.ydbio.2013.08.028 PubMed 24036310