The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage Behavior during Mycobacterial Infection in Zebrafish.
Walton EM, Cronan MR, Beerman RW, Tobin DM
PLoS One. 2015 Oct 7;10(10):e0138949. doi: 10.1371/journal.pone.0138949. eCollection 2015. PubMed
Walton EM, Cronan MR, Beerman RW, Tobin DM
PLoS One. 2015 Oct 7;10(10):e0138949. doi: 10.1371/journal.pone.0138949. eCollection 2015. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 70052 | p5E-mfap4 | 5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes. |
| 135210 | p3E tdTomato | Red fluorescent tag |
| 135211 | p3E p2a-tomato | Red fluorescent tag |
| 135214 | pDEST mfap4:tdTomato | Red tagged macrophages |
| 135217 | pDEST mfap4:icre-p2a-tomato | Cre coexpressed with tdTomato in macrophages |
| 135218 | pDEST mfap4:turquoise2 | Cyan tagged macrophages |
| 188700 | p3e tdTomato | Gateway 3' element encoding tdTomato |
| 188701 | pDEST tol2 UbB polyA | Gateway destination vector containing the zebrafish ubiquitin B polyadenylation sequence |
| 188702 | p3e UbB polyA | Gateway 3' element encoding the ubiquitin B polyadenylation sequence |