Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #78686)


Item Catalog # Description Quantity Price (USD)
Plasmid 78686 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2600
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    acta2 promoter
  • Alt name
    actin, alpha 2, smooth muscle, aorta
  • Alt name
    alpha smooth muscle actin
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    acta2 (a.k.a. wu:fb63d03)
  • Promoter acta2

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The entire intron 1 was inserted upstream of 300 bp of proximal acta2 promoter to make a compact promoter/enhancer construct that could easily be used for transgenesis.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5E-acta2 was a gift from Sarah Childs (Addgene plasmid # 78686 ; ; RRID:Addgene_78686)
  • For your References section:

    An alpha-smooth muscle actin (acta2/alphasma) zebrafish transgenic line marking vascular mural cells and visceral smooth muscle cells. Whitesell TR, Kennedy RM, Carter AD, Rollins EL, Georgijevic S, Santoro MM, Childs SJ. PLoS One. 2014 Mar 3;9(3):e90590. doi: 10.1371/journal.pone.0090590. eCollection 2014. PONE-D-13-28889 [pii] PubMed 24594685