acta2_mCherry_pA_pDestTolpA2
(Plasmid #78685)

• Purpose: acta2:mCherry transgenesis construct
• Depositing Lab: Sarah Childs
• Depositing Organization: University of Calgary
  Located in Canada
• Publication: Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 10.1371/journal.pone.0090590. eCollection 2014.

Backbone

• Vector backbone: pDestTol pA2
• Backbone manufacturer: Koichi Kawakami/ Chi-Bin Chien
• Backbone size w/o insert (bp): 2417
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: acta2 promoter
• Alt name: actin, alpha 2, smooth muscle, aorta
• Alt name: alpha smooth muscle actin
• Species: D. rerio (zebrafish)
• Insert Size (bp): 3428
• GenBank ID: NM_212620.1
• Entrez Gene: acta2 (a.k.a. wu:fb63d03)
• Promoter: acta2
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Tol2-F
• 3′ sequencing primer: mCherry-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We gateway cloned this combination using our acta2 promoter and components from Tol2kit.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 78685-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 78685-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.