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Addgene

tol2-mpeg1-mcherry
(Plasmid #58935)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58935 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Tol2
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 6124
  • Modifications to backbone
    Inserted mpeg1-mcherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mpeg1-mcherry
  • Insert Size (bp)
    2800
  • Promoter mpeg1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site xhoI (not destroyed)
  • 3′ cloning site xbaI (not destroyed)
  • 5′ sequencing primer Gtgaaagtacaagtacttagggaaaa
  • 3′ sequencing primer gtgtggaattgtgagcggataac
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tol2-mpeg1-mcherry was a gift from Anna Huttenlocher (Addgene plasmid # 58935 ; http://n2t.net/addgene:58935 ; RRID:Addgene_58935)