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acta2_GFP_pA_pDestTolpA2
(Plasmid #78684)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 78684 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDestTol pA2
  • Backbone manufacturer
    Koichi Kawakami/ Chi-Bin Chien
  • Backbone size w/o insert (bp) 2417
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    acta2 promoter
  • Alt name
    actin, alpha 2, smooth muscle, aorta
  • Alt name
    alpha smooth muscle actin
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    3494
  • GenBank ID
    NM_212620.1
  • Entrez Gene
    acta2 (a.k.a. wu:fb63d03)
  • Promoter acta2

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    We gateway cloned this combination using our acta2 promoter and components from Tol2kit.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    acta2_GFP_pA_pDestTolpA2 was a gift from Sarah Childs (Addgene plasmid # 78684 ; http://n2t.net/addgene:78684 ; RRID:Addgene_78684)
  • For your References section:

    An alpha-smooth muscle actin (acta2/alphasma) zebrafish transgenic line marking vascular mural cells and visceral smooth muscle cells. Whitesell TR, Kennedy RM, Carter AD, Rollins EL, Georgijevic S, Santoro MM, Childs SJ. PLoS One. 2014 Mar 3;9(3):e90590. doi: 10.1371/journal.pone.0090590. eCollection 2014. PONE-D-13-28889 [pii] PubMed 24594685