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PresentER-SIINFEKL (GFP)
(Plasmid #102944)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102944 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PresentER
  • Backbone size w/o insert (bp) 8300
  • Total vector size (bp) 8321
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin ; GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SIINFEKL
  • Alt name
    Chicken Ovalbumin H2-Kd epitope 257-264
  • Alt name
    OVA257-264
  • Alt name
    OVAL
  • Species
    G. gallus (chicken), Synthetic
  • Insert Size (bp)
    24
  • Mutation
    OVA257-264
  • Entrez Gene
    OVAL (a.k.a. OVA, SERPINB14)
  • Promoter LTR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (not destroyed)
  • 3′ cloning site SfiI (not destroyed)
  • 5′ sequencing primer GTT CGA CCC CGC CTC GAT CC
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/09/22/267047 for bioRxiv preprint. Please note that the Addgene sequencing result identifies a V163A mutation within EGFP that does not affect fluorescence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PresentER-SIINFEKL (GFP) was a gift from David Scheinberg (Addgene plasmid # 102944 ; http://n2t.net/addgene:102944 ; RRID:Addgene_102944)
  • For your References section:

    Rejection of immunogenic tumor clones is limited by clonal fraction. Gejman RS, Chang AY, Jones HF, DiKun K, Hakimi AA, Schietinger A, Scheinberg DA. Elife. 2018 Nov 30;7. pii: 41090. doi: 10.7554/eLife.41090. 10.7554/eLife.41090 PubMed 30499773