164,599 results

1. pLenti PGK GFP Puro (w509-5)

   Plasmid

   #19070

   Purpose

   3rd gen lentiviral eGFP expression vector, PGK promoter, Puro 

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-2NLS-Cas9-6XMS2

   Plasmid

   #166033

   Purpose

   Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0079 RESCUE-S

   Plasmid

   #130662

   Purpose

   pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

   Insert

   dRanCas13b-ADAR2dd
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. bMS.346

   Bacterial Strain

   #220588

   Purpose

   Increase editing efficiency of retron and oligo recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Bacterial Resistance

   None

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

5. pMXs-Klf4

   Plasmid

   #13370

   Purpose

   Retroviral expression of mouse Klf4

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   Kruppel-like factor 4 (gut) (Klf4 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXG237

   Plasmid

   #141281

   Purpose

   ATF4 translational reporter

   Depositor

   Martin Kampmann

   Article

   Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

   Insert

   uORF1/2 of ATF4 followed by mApple
   

   Use

   Lentiviral

   Tags

   mApple

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set B

   Pooled Library

   #92380

   Purpose

   CRISPRa

   Depositor

   John Doench, David Root

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Use

   Lentiviral

   Available since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pKLV2-EF1a-Cas9Bsd-W

   Plasmid

   #68343

   Purpose

   Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-Cas9 cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Cas9 fused with Flag-tag and a NLS at the N termi…

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-AQP9

   Plasmid

   #48808

   Purpose

   Express human AQP9, which induces filopodia formation

   Depositor

   Kenneth Jacobson, Vesa Loitto

   Article

   Loitto et al Exp Cell Res. 2007 Apr 15;313(7):1295-30

   Insert

   AQP9 (AQP9 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDA_052

    Plasmid

    #136474

    Purpose

    Lentiviral expression of AsCas12a gRNA

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    Puromycin resistance
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF2069

    Plasmid

    #141902

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

    Plasmid

    #207863

    Purpose

    AAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHLSec2-FN-YPet

    Plasmid

    #65421

    Purpose

    Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7

    Depositor

    Harold Erickson

    Article

    Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9

    Insert

    human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
    

    Use

    Tags

    YPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAA3.stelN

    Plasmid

    #112011

    Purpose

    Expresses TelN enzyme

    Depositor

    Stephen Harrison

    Article

    Jenni et al Science. 2018 May 4;360(6388):552-558. d

    Insert

    TelN
    

    Use

    Tags

    strep and his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBABE-MCS(extended)-Puro (empty vector)

    Plasmid

    #172603

    Purpose

    Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    The multiple cloning site (MCS) has been edited/e…

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-FKBP-MTM1

    Plasmid

    #51614

    Purpose

    Recruitable PtdIns3P/PtdIns(3,5)P2 phosphatase

    Depositor

    Tamas Balla

    Article

    Hammond et al J Cell Biol. 2014 Apr 7.

    Inserts

    FKBP
    MTM1 (MTM1 1812, Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG

    Plasmid

    #129632

    Purpose

    Visualization of Akt and ERK activity

    Depositor

    Dorus Gadella

    Article

    Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

    Insert

    PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4360) (AAV PHP.eB)

    Viral Prep

    #214856-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4360) (#214856). In addition to the viral particles, you will also receive purified AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4360) plasmid DNA. minBG-driven expression of jGCaMP8s in striatal direct pathway medium spiny neurons (D1 MSNs). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Promoter

    Tags

    None

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

19. pLenti_EF1a_Flag_EGFP

    Plasmid

    #183117

    Purpose

    Expresses flag-tagged EGFP in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. SP6-VEE-GFP

    Plasmid

    #58976

    Purpose

    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Insert

    EGFP
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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